Isolation and characterization of hydrocarbon degrading. Hydrocarbon oxidizing bacteria have been isolated from a variety of terrestrial and aquatic environments, using both enrichment and direct plating techniques. Most petroleum hydrocarbons encountered in the environment are ultimately degraded or metabolized by indigenous bacteria because of their energetic and carbon needs for growth and reproduction, as well as the requirement to relieve physiological stress caused by the presence of petroleum hydrocarbons in the microbial bulk environment hazen et al. The diversity of indigenous bacteria in sediments from several sites in the elizabeth river virginia able to degrade multiple polycyclic aromatic hydrocarbons pahs was investigated by the use of classical selective enrichment and molecular analyses. Microbes behind the scenes of the biodegradability of plastic. In the same sand samples for which detailed chemical analysis was performed, mpn counts of cultivatable hydrocarbondegrading bacteria in oilcontaminated sands 2.
Feb 06, 20 the present study describes the isolation and identification of total heterotrophic bacterial thb population and hydrocarbon degrading bacterial hdb population, identification of the efficient degrader, its molecular characterization and phylogenetic analysis based upon 16s rna gene sequencing from the samples collected in ennore creek. Reconstructing metabolic pathways of hydrocarbondegrading. The in situ metabolism of oildegrading bacteria is likely to be limited by a number of environmental parameters, including temperature, the availability of oxygen and major nutrients, oil hydrocarbon content, and weathering or dispersal of the oil. Isolation of hydrocarbon degrading bacteria following the strategy designed in our work to isolate hydrocarbon degrading bacteria both with high growth and biological activity, 39 purified bacterial isolates were recorded amongst hundreds of isolated ones at the first step of the program. Pdf identification of hydrocarbon degrading bacteria by. Diversity of diesel degrading bacteria from a hydrocarbon. Distribution of hydrocarbondegrading bacteria in the soil. However, cultures have not been preserved, or attempts to reproduce hydrocarbon oxidation by desulfovibrio strains failed 1, 2. Petroleum hydrocarbons can be degraded by microorganisms such as bacteria, fungi, yeast and microalgae 3, 4. To characterize polycyclic aromatic hydrocarbon pah.
Source of isolation the source for bacterial isolation consisted of wastewaters contaminated with. Marine oil spill bioremediation by immobilized and free cells. Department of microbiology, rishi biotech, mumbai400053, india corresponding author a b s t r a c t experiments were performed in low and high 1% and 10% concentration of crude bacillus introduction. Proximate analyses were carried out on the best carrier materials. Hydrocarbondegrading bacteria are important resources for use in phytoremediation applications. This study isolated pahdegrading bacteria from enriched cultures of spent automobile engineoil. Alcanivorax borkumensis was the first hcb to have its genome sequenced. Aliphatic hydrocarbons decreased in comparison with those of the abiotic control regarding the nonresolved background cycloalkanes, resins and asphaltenes. Microbiological nitrogen fixation is an activity that requires large amounts of energy. Hydrocarbondegrading bacteria and the bacterial community response in gulf of mexico beach sands impacted by the deepwater horizon oil spill joel e. These isolates were purified from soil samples on the basis of their colony morphology, texture, growth, temperature and hydrocarbon utilization ability. In previous studies, two hydrocarbon degrading sulfatereducing bacteria, strains pp31 t and pl12 t, were obtained from oilpolluted marine sediments of shuaiba, kuwait. Hydrocarbon degrading bacteria medina agriculture products.
Pdf characterization of hydrocarbon degrading bacteria. Proliferation of hydrocarbondegrading microbes at the. Interest in possible hydrocarbon oxidation by sulfatereducing bacteria arose from their frequent presence in oil production. Aug 17, 2017 at present, many types of hydrocarbon degrading bacteria that can utilise different types of hydrocarbons as carbon and energy sources in metabolism exist in the marine environment 1, 2.
The isolation of hydrocarbon degrading bacteria in topsoil and subsoil samples of selected mechanic workshops located in the benin metropolis was carried out. Microbial biodegradation is the use of bioremediation and biotransformation methods to harness the naturally occurring ability of microbial xenobiotic metabolism to degrade, transform or accumulate environmental pollutants, including hydrocarbons e. Petroleum hydrocarbon degrading bacteria were obtained after enrichment on crude oil as a chocolate mousse in a continuous supply of indonesian seawater amended with nitrogen, phosphorus and iron nutrients. Several bacteria are even known to feed exclusively on hydrocarbons 39. Pdf isolation, identification and characterization of. The deepwater horizon oil spill produced large subsurface plumes of dispersed oil and gas in the gulf of mexico that stimulated growth of psychrophilic, hydrocarbon degrading bacteria. Petroleum hydrocarbondegrading bacteria are ubiquitous in nature and can utilize these compounds as sources of carbon and energy. The isolated hydrocarbon degrading bacterial strains were analyzed with gram staining, motility test, spore.
Bacteria that cause for biodegradable plastic usually are the dominant hydrocarbon degraders in landfill environments. Because of their diverse molecular structures, it is difficult. Contaminated soil samples were collected from warri refinery jetty while water sample was collected from. Anaerobic degradation of oil hydrocarbons by sulfatereducing. During screening for biosurfactantproducing, nalkanedegrading marine bacteria, six heterotrophic bacterial strains were isolated from enriched mixed cultures, obtained from sea watersediment samples collected near the isle of borkum north sea, using mihagols c14,15nalkanes as principal carbon source. They were resistant to heavy metals such as lead, iron, zinc, cobalt and mercury.
Twentythree hydrocarbondegrading bacteria strains were isolated from gas station leakingcontaminated groundwater. Pdf petroleum hydrocarbondegrading bacteria for the. Potential of polycyclic aromatic hydrocarbondegrading. Altogen labs has developed active bioremediation technology based on natural oildegrading bacteria for largescale remediation of hydrocarboncontaminated water and soil. Torrent semiconductor sequencing, dna nanoball sequencing, heliscope single. Furthermore, the dominant petroleum hydrocarbon degrading bacteria were shifted from arcobacter in production water to pseudomonas after the first subculture and then to bacillus after the sixth subculture. Petroleum hydrocarbon degrading bacteria are ubiquitous in nature and can utilize these compounds as sources of carbon and energy. Isolation, screening and activity of hydrocarbondegrading. Isolation of hydrocarbon degrading bacteria from soils.
Biodegradation of crude oil in contaminated soils by free and. Hydrocarbondegrading microorganisms are ubiquitous in the marine environment 27, 76, and biodegradation. Hydrocarbons are degraded primarily by bacteria and fungi. Engineered clays as sustainable oil dispersants in the. Hydrocarbon degrading bacteria are known as hydrocarbonoclastic bacteria hcb. Hydrocarbons, enrichment culture technique, oil degrading bacteria, gravimetric analysis, biochemical tests. For these facultative hydrocarbon degraders the occasional supertanker oil spill forms an occasional carbon banquet. Genomic diversity of two hydrocarbondegrading and plant. One of the most important features of hydrocarbon degrading bacteria is the ability.
Characteristic assessment of dieseldegrading bacteria. The biodegradation of crude oil in soil by immobilized cells was compared with that by free cells at three. Succession of hydrocarbondegrading bacteria in the aftermath. Powtoon is a free tool that allows you to develop cool animated clips and animated presentations for your website, office meeting, sales pitch, nonprofit fundraiser, product launch, video resume. Hydrocarbon degrading bacteria are important resources for use in phytoremediation applications. We tracked succession of plume bacteria before, during and after the 83day spill to determine the microbial response and biodegradation potential throughout the incident. Medina hydrocarbon degrading bacteria consists of known nonpathogenic microbial strains that degrade petroleum hydrocarbons in solid and aqueous environments.
Microorganisms and culture conditions petroleum hydrocarbon degrading bacteria, bacillus cereus s1, was isolated from oilpolluted sea. Jyothi kommula, vit university, for the award of the degree of master of science, is a record of bonafide work carried. Results the result of the bacterial count showed 38. In the present study, we demonstrated that hydrocarbonoclastic bacteria isolated from all over kuwait exhibited diazotrophic potential, as well hydrocrbonoclastic actual. The principal hydrocarbon degrading microorganisms are bacteria and fungi.
Sufficient time is needed to synthesize hydrocarbon degrading enzymes because of the requirements of. Hydrocarbondegrading bacteria were isolated from 2% hydrocarbon enriched sample collected from vehicular repairing stations, garages and petrol bunk. Genomewide surveys of these isolates suggest that the oyster microbiome, which remains largely understudied, has a strong potential to degrade. Temperatures in this subtropical environment remain optimal for microbial growth throughout the summer, and oxygen profiling indicates that the pensacola beach sands remained aerobic throughout the study period with oxygen concentrations at all. The indigenous hdb were widely distributed throughout the soil environment and ranged from 3. Enrichment cultures containing naphthalene, phenanthrene, fluoranthene, or pyrene as a sole carbon and energy source were monitored by. One potent gram negative bacillus bacteria was isolated on the basis of petroleum hydrocarbon degradation efficiency and identified as pseudomonas sp. Isolation and identification of petroleum hydrocarbon. Screening and isolation of a hydrocarbon degrading. For these facultative hydrocarbon degraders the occasional supertanker oil spill forms. Hydrocarbons are generally colorless and hydrophobic with only weak odors. Bacteria are the most active agents in petroleum degradation, and they work as primary degraders of spilled oil in environment 37, 38.
Proliferation of hydrocarbondegrading microbes at the bottom. Ringim mahi saidu, gumel ahmad mohammed, shiaka gimba peter, isolation and molecular identification of hydrocarbon degrading bacteria from contaminated soil in mechanic village dutse, jigawa state, international journal of microbiology and biotechnology. Genes free fulltext genomic diversity of two hydrocarbon. Isolation and screening of hydrocarbon degrading bacteria from soil near kadi gujarat region. Bacteria displaying such capabilities are often exploited for the. Petroleum hydrocarbondegrading bacteria for the remediation. These gramnegative, aerobic, rodshaped bacteria use a limited number of organic. This study aimed to isolate and identify hydrocarbon degrading bacteria and.
Department of microbiology, rishi biotech, mumbai400053, india corresponding author a b s t r a c t experiments were performed in low and high 1% and 10% concentration of. Immobilization was by direct adsorption of the isolates. Succession of hydrocarbondegrading bacteria in the. The optimum ph for hydrocarbon degradation by flavobacterium spp. Pdf isolation and screening of hydrocarbon degrading. Pdf characterization of hydrocarbon degrading bacteria isolated. An unexpected enrichment for hydrocarbon degrading bacteria, along with an increase in the abundance and transcription of hydrocarbon degrading genes, was found at depths near the trench bottom 10,400m and 10,500 m. Adaptation is brought about by selective enrichment of hydrocarbonutilizing microorganisms and amplification of the pool of hydrocarboncatabolizing genes. Source of isolation the source for bacterial isolation consisted of wastewaters contaminated with used motor oil, diesel and lubricating oils. Directional culture of petroleum hydrocarbon degrading. Hydrocarbondegrading bacteria were isolated from crude oil contaminated sites in gio and kdera, rivers state, nigeria using enrichment method.
Isolation and characterization of hydrocarbondegrading. Viability of hydrocarbondegrading bacterial consortium immobilized on different carriers was studied. Based on this information, we experimentally tested and confirmed the hydrocarbon degradation activity of. The bacterial strains were isolated by inoculating the soil and water sample into the prepared nutrient agar medium which is autoclaved and added 1. Pdf with the sharp increase in population and modernization of. Bioremediation of petroleum based contaminants with biosurfactant. Hundred milliliters of the cell free media was shaken for foam formation and. Comparative study of five polycyclic aromatic hydrocarbon degrading bacterial strains isolated from contaminated soils. Incubation of the isolated nonvirulent strains in bushnellhaas bh medium supplemented with hydrocarbon, showed their ability to survive on hydrocarbon thereby reducing the total organic.
Aerobic bacteria degrading both n alkanes and aromatic. Biodegradation is a cost effective and ecofriendly treatment for oilcontaminated materials by the use of microorganisms. Comparative studies on bacterial consortia for hydrocarbon. Green,1,3 gina freyer,1,2 andy canion, jonathan delgardio, 1nikita. Draft genome sequences of oysterassociated pseudomonas stutzeri strain mf28, p.
These aerobic, gramnegative rods are motile by means of. Screening of hydrocarbon degrading bacteria isolated from oil contaminated soil article pdf available june 2019 with 192 reads how we measure reads. These strains grow by using a limited number of organic compounds, including the aromatic hydrocarbons naphthalene, phenanthrene, anthracene, and toluene, as sole carbon sources. Isolation and identification of effective hydrocarbon degrading bacteria from diesel polluted soil doi. Mineral clay particles present in abundance in marine environments adsorb at oilwater interfaces forming stable emulsion droplets. International journal of current microbiology and applied. Isolation and identification of hydrocarbon degrading. Pnp degrading bacteria and this item was submitted in to occur in microform, scholars, gejun. Products from the incomplete metabolism of pyrene by. More than 100 strains were isolated for their ability to use fluoranthene as the sole carbon and energy source. Viability of hydrocarbondegrading bacterial consortium. This study was aimed at bacterial degradation of polycyclic aromatic hydrocarbons pah in untreated refinery effluent.
Individually immobilized and surfacemodified hydrocarbon. Enrichment, isolation and identification of hydrocarbon degrading bacteria udgire m. Media used in this study were mineral salts medium msm, which contained per liter. Petroleum hydrocarbondegrading bacteria and the type of petroleum. Similarly, in preliminary study 57 oil degrading bacteria were isolated from oil impregnated soil in search of potent hydrocarbon degrader 14. Characterization of aromatic hydrocarbon rading bacteria. We describe the carbonization of these clays using the sustainable biopolymer, chitosan, to optimize wetting. Adaptation by prior exposure of microbial communities to hydrocarbons increases hydrocarbon degradation rates. Three heterotrophic bacterial strains were isolated from different locations in puget sound, washington, by using biphenyl as the principal carbon source. Enrichment and isolation of petroleum hydrocarbon degrading bacteria petroleum hydrocarbon degrading bacteria were isolated from oil contaminated soil as described above. With the sharp increase in population and modernization of society, environmental pollution resulting from petroleum hydrocarbons has increased, resulting in an urgent need for remediation. Among the true marine marinobacter genus alteromonadaceae family associated with n alkane transformation, there are several strains metabolizing both saturated.
Hydrocarbons from which one hydrogen atom has been removed are functional groups called hydrocarbyls. Although bacteria able to grow on aliphatic and aromatic hydrocarbons are found in many genera, the genera alcanivorax appear to be special because these bacteria are specialized for. Anaerobic hydrocarbondegrading sulfatereducing bacteria. Department of chemistry, rochester institute of technology, rochester, ny.
Isolation and identification of effective hydrocarbon. Enrichment, isolation, and phylogenetic identification of. The hydrocarbon degrading bacterial types and numbers were found to be lesser than their heterotrophic counterparts. The highest and lowest bacterial counts in topsoil samples were found out to be 2. An unexpected enrichment for hydrocarbondegrading bacteria, along with an increase in the abundance and transcription of hydrocarbondegrading genes, was found at depths near the trench bottom 10,400m and 10,500 m. Characterization of hydrocarbon degrading bacteria isolated from hydrocarbon contaminated soil. Sample collection sediment samples were collected from the munakkal beach trichur dist, kerala located on the west coast of india immediately after the major event tsunami in 2004. Isolation and characterization of hydrocarbondegrading bacteria. Restoration of polycyclic aromatic hydrocarbon pah polluted sites is presently a major challenge in agroforestry.
Particle stabilized emulsions provide an environmentally friendly alternative to the chemical dispersion of oil in the event of a spill over water. Kleindienst s 2012 hydrocarbondegrading sulfatereducing bacteria in marine hydrocarbon seep sediments. Isolation and identification of hydrocarbon degradation bacteria. Supplied in a dry bran mixture, microorganisms are activated when mixed with water. Several bacteria are even known to feed exclusively on hydrocarbons yakimov et al. Botanyenvironmental pollution,mbamarketing green apple environmental technologies 287patpadganj industrial area, delhi110 092india brief facts bacteria generally degraders in aquatic systems such as oceans, ponds and other water reservoir. Identification of hydrocarbon degrading bacteria the bacteria isolated were identified based on physical characterization and the biochemical tests outlined in bergeys manual of determination bacteriology holt et al. Many indigenous microorganisms in water and soil are capable of degrading. Simultaneous degradation of atrazine and phenol by pseudomonas sp. Jun, 2018 interestingly, marine hydrocarbon degrading bacteria belonging to gammaproteobacteria also revealed the broadened preferences towards structurally divergent groups of hydrocarbons. Isolation and characterization of hydrocarbon degrading bacteria from environmental habitats in western new york state, masters thesis in chemistry, 2005.
Pdf table of contents submit biotechnology research international. Sep 14, 2016 powtoon is a free tool that allows you to develop cool animated clips and animated presentations for your website, office meeting, sales pitch, nonprofit fundraiser, product launch, video resume. Wholegenome sequences of five oysterassociated bacteria. Frontiers petroleum hydrocarbondegrading bacteria for. Hydrocarbondegrading bacteria enriched by the deepwater horizon oil spill identified by cultivation and dnasip. The level of petroleum hydrocarbon degradation was determined by gravimetric assay at each 5 days interval. They also possess diverse metabolic pathways which is not seen in fungi which allows them. Despite its toxicity, a considerable fraction of petroleum oil entering marine systems is eliminated by the hydrocarbondegrading activities of microbial communities, in particular by a recently discovered group of specialists, the hydrocarbonoclastic bacteria hcb. Also, samples amended with cp were found to have more types and higher numbers of heterotrophic and hydrocarbon degrading bacteria than the samples without amendment controls. A realtime pcr quantification method for indigenous hydrocarbondegrading bacteria hdb carrying the alkb gene in the soil environment was developed to investigate their distribution in soil. Floodgate 36 listed 25 genera of hydrocarbon degrading bacteria and 25 genera of hydrocarbon degrading fungi which were isolated. The occurrence of polycyclic aromatic hydrocarbons pah in refinery effluents is of great concern globally due to its persistence, recalcitrance and carcinogenicity. Study on the biodegradation of crude oil by free and. Pdf oceanobacterrelated bacteria are important for the.
Necessity of consortium with broad enzymatic capacities for degradation of complex hydrocarbon pollutants such as crude oil or diesel fuel was also reported. The goal of this project was to evaluate the effects of test conditions and bacterial growth conditions on the results and precision of the math test using a single species of hydrocarbon degrading bacteria. Yet, for many hydrocarbonoclastic strains the genetic information regarding pollutant degradation and detoxification has not been thoroughly revealed. Consequently, microorganisms with pahdegradation ability and soil fertility improvement attributes are sought after in order to achieve sustainable remediation of polluted sites. The observation that the oceans are not covered with an oily layer is a testimony to the activity of the hydrocarbon. During screening for biosurfactantproducing, nalkane degrading marine bacteria, six heterotrophic bacterial strains were isolated from enriched mixed cultures, obtained from sea watersediment samples collected near the isle of borkum north sea, using mihagols c14,15nalkanes as principal carbon source. Isolation and molecular identification of hydrocarbon. Pdf screening of hydrocarbon degrading bacteria isolated. They also posses diverse metabolic pathways that are not present in fungi which allows them to utilize most recalcitrant petroleum hydrocarbons. Petroleum hydrocarbondegrading bacteria for the remediation of.
The present study describes the isolation and identification of total heterotrophic bacterial thb population and hydrocarbon degrading bacterial hdb population, identification of the efficient degrader, its molecular characterization and phylogenetic analysis based upon 16s rna gene sequencing from the samples collected in ennore creek. Fifty seven 57 bacteria degrading crude oil from the hydrocarbon polluted sites of gasoline and diesel spilled gas stations of coimbatore, india have been isolated. The function of hydrocarbon degrading bacteria mainly depends on their hydrocarbon degrading enzymes, the expression and activity of which are closely related to the physiological activity of bacteria mukherjee et al. In organic chemistry, a hydrocarbon is an organic compound consisting entirely of hydrogen and carbon 620 hydrocarbons are examples of group 14 hydrides.
Hydrocarbondegrading microbial communities with high tolerance to and high degrading ability of. Aromatic hydrocarbons such as benzene, hexane, toluene, naphthalene and xylene degrading bacteria such as flavobacterium spp. Microbial degradation of hydrocarbons in the environment. Frontiers petroleum hydrocarbondegrading bacteria for the. Dec 03, 2018 petroleum hydrocarbon degrading bacteria. This bacterium is of particular interest as it is a cosmopolitan marine bacterium that blooms in oil. Crude oil and petroleum is a complex mixture of hydrocarbons, which cause widespread environmental contaminants following a spill. The heteroatom groups in the crude oil were biodegraded simultaneously with normal alkanes and alkyl cyclohexanes. Screening and isolation of a hydrocarbon degrading bacteria by enrichment culture technique. The hydrocarbo n degrading bacterial counts for the. Pdf polycyclic aromatic hydrocarbons pahs are of particular concern due. Hydrocarbondegrading bacteria and the bacterial community. They had been reported as organisms capable of anaerobic degradation of pxylene and nalkanes, respectively. They were related to alcanivorax and marinobacter strains, which are ubiquitous petroleum hydrocarbon degrading bacteria in marine environments, and to oceanobacter kriegii 96.
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